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The recently digitised medieval manuscripts from the National Library are relatively varied in their content. Apart from the most common theological and preaching texts and liturgical codices, they also comprise ancient literature (VIII H 24), rhetorical (VIII H 22), medical (VIII H 34), historiographical (XIII D 7) or hagiographical (XIV E 8) texts. In the Middle Ages, codices XIII D 7 and XIII F 12 formed part of the library of Charles College of the Prague university. They are included in its earliest library inventory. The manuscripts likewise come from other large medieval institutional libraries: the Augustinian monastery in Třeboň and the Cistercian monastery in Zlatá Koruna. Czech-language manuscripts are represented by three volumes of the Bible, illuminated manuscripts mainly by the prayer book VI G 24.

The first digitised document from the collections of the Museum of the Vysočina Region in Havlíčkův Brod is an evangelical postil by Martin Philadelphus Zámrský, which was printed in Leipzig in 1602. The incompletely preserved early printed book was also restored before the digitisation.

The Museum of the Brno Region Muzeum has provided access to another four medieval manuscripts from the library of the Benedictine monastery in Rajhrad in 2015. The missals R 397 and R 398 as well as the exegesis of the New Testament and Biblical prologues R 18 were created in the second half of the 14th century, the Czech New Testament complemented by other texts R 390 was written in the first half of the 15th century. With the exception of the New Testament, all the manuscripts are decorated to varying degrees.

The newly digitised items from the collections of the Music Department of the National Library of the CR comprise 32 manuscripts and printed items. They mostly include handwritten copies of short scores from the collection of Josef Cyril Strachota, composed by W. A. Mozart, V. Pichl, F. X. Pokorný and J. L. Oehlschlägel. A longer manuscript is placed under the shelf mark XVI H 8 – it contains compositions collected by Franz Eduard Tuna, partly his own works. The printed items come from the 16th and 17th centuries and include collections of polyphonic compositions.