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Two manuscripts from the 14th century which belonged to the monastery of the Augustinian Canons in Roudnice nad Labem in the Middle Ages were digitised from the collections of the National Museum Library. The first (XIII A 1) contains a collection of letters of Pope Gregory I, the second (XIII A 3) a commentary on the Gospels of Matthew and Mark by Thomas Aquinas.

The manuscripts of the National Library that have recently been made accessible can be divided into several groups. The largest of them comprises medieval codices mostly of Czech origin, whose content is quite varied: there are preaching and theological, but also legal, liturgical, medical, mathematical and astronomical texts. The most interesting manuscript in German (shelf mark XVI) are the town statutes of Horní Slavkov. The digitised items further included two volumes of the Memoirs of Vilém Slavata of Chlum and Košumberk from the collections of the Prague Lobkowicz Library and various copies of earlier historical sources from the Thun-Hohenstein Library in Děčín. Two manuscripts from the 17th and 18th centuries were made accessible also from collections of the Slavonic Library.

The greatest part of digitised manuscripts of the Military History Institute in Prague is formed by reports of Prince Albert Casimir August of Saxony, Duke of Teschen, on various military conflicts of the last quarter of the 18th century, accompanied by extensive map material and tabular overviews. Smaller volumes contain copies of military orders, reports on military campaigns and treatises on ammunition casting.

Another part of the manuscripts from the collections of the National Library that have been made accessible contains mainly modern handwritten copies of earlier historical sources coming from the Thun-Hohenstein library in Děčín (shelf mark XIX); handwritten copies of works by Bohuslav Balbín can be found in the two-volume manuscript XXIII.C.40. The medieval codices that have recently been digitised are of Czech origin and were written in the second half of the 14th century and at the beginning of the 15th century; in terms of content, they are an assortment of sermonic, mystical, natural-science, theological and philosophical works.