The first part of the manuscripts digitised from the collections of the National Library of the Czech Republic in 2022 comprises 27 medieval codices placed under the shelf marks I–VI. Most of the manuscripts are of Czech origin and were written in the 14th and 15th centuries. In terms of content, they mainly include various theological writings and collections of sermons. Among the works of Czech authors, access has been provided to the commentary of Jan Rokycana on two letters of the Apostle Paul (shelf mark IV A 24). Some volumes also contain works on the natural sciences – for instance a treatise on medicines, probably by Nicholas of Salerno (III E 13), a medical compendium (I G 23) and the astrological work Liber introductorius ad iudicia stellarum by Guido Bonatti de Forlivio (IV B 10). Liturgical manuscripts are represented, for example, by a 14th-century hymnal of Friars Minor (VI C 20b), a Cistercian missal from the turn of the 14th century (I E 10) and a book of sequences from the turn of the 16th century (VI C 15). A work important for the history of German literature is the collection I C 40, which contains, among other things, Heinrich Seuse’s Büchlein der Ewigen Weisheit, Irmhart Öser’s translation of Rabbi Samuel’s letter to Rabbi Isaac, and the so-called ‘Münchner Apostelbuch’.